Topics for Chapter 3: Amino acids, peptides and protein purification

 

Amino acid properties

Stereochemistry

Side chain classes

Absorbance

Acid/base

Electric charge

 

Peptide bonds

Poly peptides

Protein primary structure

 

Protein purification

The importance of an assay

Salt fractionation and dialysis

Gel electrophoresis

Column chromatography

Molecular sieves

Ion exchange

Affinity

 

Determining the amino acid sequence of proteins

Edman degradation

The automated sequenator

Mass spectrometry

 

Evolution of proteins

Relatedness of sequences

Conserved and variable residues